Alkaline Phosphatase Rapid Test Kit

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Alkaline Phosphatase Rapid Test Kit

SKU: EC0035 Category:

Alkaline Phosphatase (ALP) is a heat sensitive enzyme that is naturally present in raw milk, and it can be destroyed by conventional pasteuriza-tion. This kit can determine whether the samples meet the U.S. and E.U.
pasteurization requirements of by determining the content of ALP.

Quantity

100 Tests

Shelf Life

12 Months

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Description

Assay Principles

The kit uses chemiluminescent substrate of specific enzyme to determine the activity of alkaline phosphatase in dairy products, which can quickly verify the pasteurization effect within 2 minutes. The chemiluminescent substrate can release photons under the catalysis of ALP. Within a specific concentration range of ALP, the number of released photons has a good linear relationship with ALP concentration in the sample. Combined with PureTrustTM Intelligent Fluorescence Detector, the operator can quantitatively detect ALP concentration in samples. Before testing samples, negative and positive control need to be used to calibrate and establish Pass/Fail RLU limits.

Materials Provided

Component Specification Quantity
Microtubes 2mL 100
Chemiluminescent substrates 2.2mL/vial 25
ALP positive controls 10
Microtube adapter 1
Package insert 1

Materials Recommended But Not Provided

  1. Vortex
  2. Timer
  3. PureTrustTM Intelligent Fluorescence Detector (MF1000 Premium)
  4. Mini Tube Incubator (37℃,HMG-LX)

Sensitivity

Detection limit in pasteurized milk (75℃, 15 seconds) is 20mU/L (approximately 0.002% raw milk).

Additional information

Quantity

100 Tests

Shelf Life

12 Months

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