Nitrofurans, which are effective against a wide range of bacteria, include furazolidone, nitrofurazone, nitrofurantoin and related compounds. The food and drug administration has prohibited all uses of nitrofuran drugs in food-producing animals because they pose a public health risk. Evidence indicates that the drugs may induce carcinogenic residues in animal tissues.

Use Principles

Furantoin Metabolite ELISA Kit is an indirect competitive enzyme-labeled immunoassay. The Furantoin Metabolite antigen is precoated on the wells. The precoated antigen compete the Furantoin Metabolite antibody (Antibody Solution) with Furantoin Metabolite in the sample, Furantoin Metabolite antibody binds to the Furantoin Metabolite Enzyme Conjugate. After the color development of TMB Substrate, the absorbance value of the sample is negatively correlated with the Furantoin Metabolite contained in it. Compared with the standard curve and multiplied by its corresponding dilution factor, the content of Furantoin Metabolite in the sample can be calculated.

Cross-reactivity

Furantoin Metabolite 100%

Furaltadone Metabolite <0.1%

Furacilin Metabolite <0.1%

Furazolidone Metabolite <0.1%

Materials Required But Not Provided

Devices:

—- Microplate Reader (450nm/630nm filter)

—- Oscillator

—- Vortex

—- Centrifuge

—- Homogenizer

—- Constant Temperature Incubator

—- Water Bath

—- Termovap Sample Concentrator

—- Balance (sensibility: 0.01g)

—- Graduated Pipette: 10mL

—- Rubber Bulb Syringe

—- Centrifuge Tube: 10mL or 7mL, 50mL

—- Micropipette: Single channel pipette (10-100µL, 100-1000µL) Multi-channel pipette (30-300µL)

Reagent:

—- Deionized Water

—- Ethyl Acetate (AR)

—- N-hexane (AR)

—- Methanol (AR)

—- Concentrated hydrochloric acid (HCl) (AR)

—- Sodium hydroxide (NaOH) (AR)

—- Dipotassium hydrogen phosphate trihydrate (K2HPO4·3H2O) (AR)

—- 18M concentrated sulfuric acid (H2SO4)

Provided Materials and Reagent