Dairy Qualitative and Quantitative Alpha-lactalbumin ELISA Test Kit

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Dairy Qualitative and Quantitative Alpha-lactalbumin ELISA Test Kit

SKU: SC0162 Category:

Alpha-lactalbumin is a kind of whey protein with various functions such as regulating milk production, cytolytic activity, inducing cell  growth inhibition and apoptosis, helping to relieve stress and improve work performance, and improve sleep, improve abstract visual memory.

Shelf Life

12 Months

Quantity

96 Packs

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Description

Alpha-lactalbumin is a kind of whey protein with various functions such as regulating milk production, cytolytic activity, inducing cell growth inhibition and apoptosis, helping to relieve stress and improve work performance, and improve sleep, improve abstract visual memory.

Principles

Alpha-lactalbumin ELISA Test Kit is an indirect competitive enzyme-linked immunoassay. The antibody is pre-coated on the wells, the alpha-lactalbumin in the sample competes with the enzyme conjugated antigen to bind the added alpha-lactalbumin antibody (antibody solution), which is colored by the TMB substrate. The absorbance value of the sample is negatively correlated with the content of alpha-lactalbumin, and the content of alpha-lactalbumin in the sample can be calculated by comparing with the standard curve and multiplying the corresponding dilution factor.

Cross-reactivity

α-lactalbumin…………………………………….………….100%

Materials Required But Not Provided

  1. Devices:

—- Microwell Plate Reader (450nm/630nm)

—- Oscillator / Vortex

—- Constant Temperature Incubator

—- Centrifuge tube: 7mL

—- Micropipette: Single channel pipette (10-100μL, 100-1000μL)

Multi-channel pipette (30-300μL)

  1. Reagent:

—- Deionized water

—- 18M concentrated sulfuric acid (H2SO4)

Provided Materials and Reagent

Component 96 wells
Antibody coated plate 12 × 8 wells
6 vials of standard 1mL each
Enzyme Conjugate 6mL
Antibody Solution 6mL
Substrate A 6mL
Substrate B 6mL
Concentrated Wash Buffer (10×) 40mL
Concentrated Sample Diluting Buffer 40mL
Package Insert 1
Testing Report 1
Plate cover sheet 2

Additional information

Shelf Life

12 Months

Quantity

96 Packs

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